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LF101 Electro Cell Fusion Generator

Nuclear Transfer (Cloning), Oocyte Activation, Electro Cell Fusion
Bovine, Pig, Mouse and Rabbit Cloning (Somatic Cell and Embryo Nuclear Transplant)

Plant Protoplast Fusion

Mammalian Cell Fusion




AC Pulse
Wave Shape Sine Wave
Voltage 0 - 50V (VP-P)
Frequency 0.1 - 3.9MHz Variable
Duration 0 - 99s
Shunt Resistor Greater than 50 ohm

Power : 100V (50/60Hz) 2A
Size : 370W x 430D x 160H mm
Weight : 10kg

DC Pulse
Wave Shape Square
Voltage 0 - 999V
0 - 200V (1V resolution)*
Pulse Length Range 5 - 99s (1s resolution)
Number of Pulses 1 - 99 Automatic or Manual
Pulse interval 0.1 - 9.9s
Shunt Resistor Greater than 50 ohm
Post fusion Switched On/Off

*Voltage for Cloning: 0 - 200V

Specialized setting for bovine, pig, mouse, rabbit cloning (Somatic cell and embryo nuclear transplant) will result in extremely high fusion probability (for example: More than 90% for bovine cloning).
Voltage setting with 1V resolution is possible in the specialized setting for mammalian cloning. It is suitable to output precisely the square pulse wave shape and pulse range as preset and accordingly brings about a higher rate of cell fusion probability.
Post fusion function for damage recovery of the cell can be operated with On/Off switching.
Specialized setting for plant protoplast and mammalian cell fusion can provide a desirable condition for the cell fusion, which is done with DC pulse (100V, Max.) of the precisely controlled voltage and pulse range after producing the pearl chains with DC. It can elevate the rate of hybridoma production (Cell fusion) probability.
Operation of the device is very easy because both of automatic and manual (Number of pulses: 1-99) transmitting of the pulse are possible. And the pulse interval can be selected to set between 0.1-9.9 seconds. The device can keep a constant operating condition anytime.
NEPA GENE provides a variety of electrodes for combination use with the device, which is designed to match well with its various settings through our long accumulated experiences. Combination use of the electrodes with the device enables very easy and efficient gene transfection and cell fusion.