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In Vivo & In Vitro Electroporator


In Vitro & In Vivo Transfection

Super Electroporator NEPA21





High Transfection Efficiency & Viability Without Special Buffers

◆ Features
In-Vitro Transfection

For Primary Cells, Stem Cells and Any Cell Lines
      With Electroporation Cuvettes

With Adherent Cell Electrodes   For Cells in Adherence

In-Vivo Transfection

For Mouse/Rat Brain, Eye, Muscle, Skin, Liver, Kidney, Testis, etc.

In-Utero Transfection
       For Mouse/Rat Embryo: Cerebral Cortex, Hippocampus, Spinal Cord, etc.

In-Ovo Transfection
       For Chick Embryo: Neural Tube, Limb Bud, Digestive Organ, etc.

Ex-Vivo Transfection
      For Tissue Slice, Brain Slice, Organ, Whole Embryo, etc.
*NEPA21 electroporator can cover all application range of the old-type CUY21 electroporator.





Request a Demo
See the performance of NEPA21 electroporator with your cells.


◆ Novel 3-Step Multiple Electroporation Pulse
3-step-pulse.jpg The 3-step pulse with voltage decay results in higher
transfection efficiency and lower damage WITHOUT
special buffers
Step 1 Poring Pulse Mode: High Voltage, Short Duration, Multiple Pulse(s), Voltage Decay
This poring pulse is for forming pores (small
holes) in cell membrane with minimum damage.
Step 2 Transfer Pulse Mode: Low Voltage, Logn Duration, Multiple Pulses, Voltage Dcay.
This transfer pulse is for delivering the target molecules (DNA, RNA, etc.) into cells with minimum damage..
Step 3 Polarity Exchanged Transfer Pulse
This can increase the transfection efficiency.




Applicatios with Electroporation Cuvettes
*Please click here for Electroporation Cuvette information.
NEPA21 electroporator makes it possible to achieve high transfection efficiency and viability without resourse to special buffers for primary cells, stem cell and difficult-to-transfect cells such as immune cells, blood cells, etc.
   1) Primary Cells
- BMMC  Primary Mouse Bone Marrow-Derived Mast cells
bmmc_a.jpg bmmc_b.jpg
Viability: 64% Transfon Efficiency: 78%
- Primary Mouse Neurons, Cerebral Cortex
cerebral cortex.jpg cerebral cortex_b.jpg
Viability: 80% Transfon Efficiency: 70%

- Primary Rat Bulbar Nerve Cells
bulbar nerve.jpg bulbar nerve_b.jpg
Viability: 80% Transfon Efficiency: 75%

- HASM  Primary Human Airway Smooth Muscle cells
hasm.jpg hasm-b.jpg
Viability: 90% Transfon Efficiency: 80%

- Primary Human Endometrial Stromal Cells
endometrial stromal.jpg endometrial stromal_b.jpg
Viability: 95% Transfon Efficiency: 90%


◆ Disposable Kit Comparison
Transfection Device   NEPA21 (Nepa Gene)           N (Company L)       N (Company I)  
Disposable Kit  Cuvettes ONLY
No Special Buffers
Transfection Kits
 With Special Buffers
 Transfection Kits
   With Special Buffers
Cost per
One Sample
*USA price
($120.00/50 pcs)
($160.00/10 RCT)
($435.00/25 tips)


Is your lab still using the transfection device that requires expensive disposable kits?
We hear from a lot of researchers that they are not satisfied with its high running cost.
The running cost of NEPA21 is much lower than other transfection devices!



   2) Cell Lines
V: Viability, TE: Transfection Efficiency
Cell Line V TE
Cell Line V TE

Species: Human

Raji Human Burkitt's Lymphoma cells 74% 53%
HeLa Human Cervical Carcinoma cells 95% 95%
LCL Human Lymphoblastoid cell 55% 43%
293 Human Embryonic Kidney cells 90% 90%
Nalm-6 Human B-cell Precursor Leukemia cells 65% 63%
293T Human Embryonic Kidney cells 90% 90%
KU812 Human basophilic leukaemia cells 96% 42%
TIG-7 Human Embryonic Lung Fibroblasts 89% 76%
MutuⅠ Human Burkitt Lymphoma cells 87% 91%
MRC-5 Human Embryonic Lung Fibroblasts 85% 90%
MutuⅢ Human Burkitt Lymphoma cells 54% 92%
SUSM-1 Human Fibroblasts 77% 71%

KMST-6 Human Fibroblasts 70% 60%

Species: Mouse

HT1080 Human Fibrosarcoma cells 93% 81%
MEF Mouse Embryonic Fibroblasts 80% 90%
MIA-PaCa-2 Human Pancreatic Carcinoma cells 80% 77%
L Mouse L cells 90% 65%
HepG2 Human Hepatoma cells 80% 76%
MC3T3-E1 Mouse Osteoblastic cells 40% 80%
HuH-7 Human Hepatoma cells 70% 60%
MS-1 Mouse Pancreatic Endothelial cells 90% 90%
H1299 Human Lung Cancer cells 90% 90%
RAW264.7 Mouse Macrophage-like cells 70% 56%
HSC-2 Human Squamous Carcinoma cells 93% 98%
MIN6 Mouse Pancreatic Beta cells 57% 71%
HSC-3 Human Squamous Carcinoma cells 93% 98%
MEL Mouse Erythroleukemia cells 70% 50%
OVCAR-3 Human Ovarian Carcinoma cells 90% 79%
XS106 Mouse Dendritic cells 61% 45%
RMG-1 Human Ovarian Carcinoma cells 97% 67%
mDC Mouse Myeloid Dendritic cells 79% 72%
TE-1 Human Esophageal Carcinoma cells 85% 41%
MC/9 Mouse Mast cells 76% 84%
TE-8 Human Esophageal Carcinoma cells 85% 40%
TS Mouse Trophoblast Stem cells 59% 47%
MCF-7 Human Breast Cancer cells 90% 93%

T47D Human Breast Cancer cells 90% 85%

Species: Rat

NUGC-3 Human Gastric Carcinoma cells 73% 68%
PC12 Rat Adrenal Pheochromocytoma cells 70% 50%
A549 Human Lung Adenocarcinoma cells 85% 90%
H9c2 Rat Ventricular Myoblasts 71% 82%
LNCaP Human Prostate Carcinoma cells 71% 90%
REF Rat Embryonic Fibroblasts 90% 99%
RKO Human Colorectal Carcinoma cells 80% 40%
C6 Rat Glioma cells 80% 67%
SK-N-SH Human Neuroblastoma cells 95% 95%

SH-SY5Y Human Neuroblastoma cells 60% 90%

Species: Hamster

KG-1-C Human Oligodendroglial cells 85% 60%
CHO Chinese Hamster Ovary cells 74% 90%
HaCaT Human Keratinocyte cells 40% 80%
CHO-K1 Chinese Hamster Ovary cells 95% 95%
CCD1079sk Human Skin Fibroblasts 40% 53%

iHAM-4 Human Amniotic Mesenchymal cells 59% 95%

Species: Chicken

iHAM-7 Human Amniotic Epithelial cells 70% 40%
DT40 Chicken B cells 38% 40%
HTR-8/Svneo Human Trophoblast cells 95% 67%

Jurkat Human T-cell Leukemia cells 71% 73%

Species: Carp

Namalwa Human Burkitt's Lymphoma cells 70% 75%     GTS9 Ginbuna Thymus cells 50% 30%




Applications with Adherent Cell Electrodes
By using Adherent Cell Electrode CUY900 series, now it is possible to transfer DNA/RNA directly into cells in adherence in a commercially available multi-well plate.
● Gene transfection into primary neurons (adherent) by electroporation
adherent_a.jpg adherent_b.jpg
adherent_c.jpg adherent_d.jpg
pCAGGS-EGFP plasmid was transferred into primary neurons cultured for 6 days in adherent state.
The neurons were prepared from E15 mouse cerebral cortex.
A): 2 steps pulse electroporation using the electrodes for adherent cells (CUY900-13-3-5)
B): EGFP fluorescence image of the neurons 2 days after electroporation
C): High magnification image of Figure B
     Many robust EGFP signals suggest high transfection efficiency.
D): High magnification image of Figure C (x40)
      Neurites are shown clearly.
Department of Neurochemistry, National Institute of Neuroscience




Applications with In-Vivo Electrodes
NEPA21 electroporator and CUY series in-vivo electrodes make it possible to transfer DNA/RNA into mouse/rat muscle, skin, liver, kidney, testis, ovary, brain, retina, cornea, etc.


● Transfection into brain, eye, muscle, skin, liver, kidney, testis, etc.
in vivo applications.jpg
Muscle Skin Testis Retina Brain Egg
*Please click here for detailed application information.
*Please cick here for electrode information.




Applications with In-Utero / Exo-Utero Electrodes
NEPA21 electroporator and CUY series in-utero electrodes make it possible to transfer DNA/RNA into mouse/rat embryo (Cerebral Cortex, Hippocampus, Spinal Cord, etc.)


● Transfection into mouse/rat embryonic brains
*Please click here for detailed application information.
*Please click here for electrode information.




Applications with In-Ovo Electrodes


● Transfection into chick embryos
ovo_a.jpg apli_cuy03e.jpg
Transfection into the central canal Transfection into the somites (A, E), hematopoietic system (B, F), notochord (C, G), and lateral plate mesoderm (D, H)
*Please click here for detailed application information.
*Please click here for electrode information.




Applications with Ex-Vivo Electrodes


● Transfection into brain tissues
Transfection into the brain slice.
*Please click here for detailed application information.
*Please click here for electrode information.


● Transfection into embryos in the whole embryo culture system
apli_cuy09_01.jpg apli_cuy09_02.jpg
Transfection into the developing rat cortex Transfection into the rat spinal cord
*Please click here for detailed application information.
*Please click here for electrode information.




*All features and specifications subject to change without notice.